NASC

NASC ID: N899

Name: CSLD3 (FA1228 (cDNA))

ABRC stock number: CS899

Description: The mutation was derived from the CT17 line of the INRA Versailles collection and received by J. Traas. CT17 was back-crossed to wild type WS. One of the F2 progenies, CT17-7, heterozygous for the csld3 mutation was used to obtain a homozygous line.

Donation Date: 2001-08-03

Donated by:

Ghent University Mieke van Lijsebettens

Donor Number: CT17-01

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Ws-2(N2360)

Pedigree: backcrossed once to wildtype.

Growth requirement: none

Segregation: No

Transformation method: The line was generated by Agrobacterium tumefaciens mediated T-DNA transformation by using the in planta method of Bechtold et al., 1993. CR Acad. Sci. Paris Life Sci 316, 1194-1199. The T-DNA was inserted in the last exon of the CSLD3 gene. The T-DNA vector is described by Bouchez et al., 1993. CR. Acad. Sci. Paris Life Sci 316, 1188-1193.

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	csld3-1	csld3	At3g03050

EMBL accession:

AJ297948

Phenotype

The csld# mutation causes a root hair phenotype. Root hair patterning is correct but root hair growth is affected. Root hairs are initiated at the correct positions, they grow into a bulge, subsequent elongation is severely affected visible by a reduced length.AJ297948 is a genomic clone containing the entire CSLD3 gene including the full promotor of 1200 bp.

References

Wang, X. et al. 2001. AtCSLD3, a cellulose synthase-like gene important for root hair growth in Arabidopsis. Plant Physiology 126(2): 575-586.PubMed ID: 11402188.